ABSTRACT
Objective To investigate the effect of vitamin D(VD)deficiency on the pathogenesis of Zucker diabetic fatty(ZDF)rats. Methods Male 5-6 weeks old Zucker rats were randomly divided into 4 groups according to their body weight:normal control group(ZL),VD deficient control group(ZL+VD.Def),model group(ZDF)and VD deficient model group(ZDF+VD.Def). All the rats were fed to 12 weeks of age, and body weight, food intake, water intake,urine volume,urine glucose,fasting blood glucose were measured. The glucose tolerance was tested at 11 weeks. Pancreatic samples were taken and tissue sections were examined by pathology using HE staining. Results The body weight of ZDF+VD.Def group was higher than that of the ZDF group. Drinking water and urine volume were increased earlier than the ZDF rats. The blood glucose in the ZDF +VD. Def group was increased significantly earlier than ZDF group,it is about 2 times of the ZDF group at 12 weeks. Compared with the ZDF group,the impaired glucose tolerance and islet damage were more serious than the ZDF + VD. Def group. Conclusions Vitamin D deficiency accelerates and aggravates the pathogenesis of ZDF rats,and VD deficiency may be a key factor in the pathogenesis of obese DM Type 2.
ABSTRACT
In this study, we investigated the effects of chronic aluminum (Al) exposure for 10 weeks on cell proliferation and neuroblast differentiation in the hippocampus of type 2 diabetic rats. Six-week-old Zucker diabetic fatty (ZDF) and Zucker lean control (ZLC) rats were selected and randomly divided into Al- and non-Al-groups. Al was administered via drinking water for 10 weeks, after which the animals were sacrificed at 16 weeks of age. ZDF rats in both Al- and non-Al-groups showed increases in body weight and blood glucose levels compared to ZLC rats. Al exposure did not significantly affect body weight, blood glucose levels or pancreatic β-cells and morphology of the pancreas in either ZLC or ZDF rats. However, exposure to Al reduced cell proliferation and neuroblast differentiation in both ZLC and ZDF rats. Exposure to Al resulted in poor development of the dendritic processes of neuroblasts in both ZLC and ZDF rats. Furthermore, onset and continuation of diabetes reduced cell proliferation and neuroblast differentiation, and Al exposure amplified reduction of these parameters. These results suggest that Al exposure via drinking water aggravates the impairment in hippocampal neurogenesis that is typically observed in type 2 diabetic animals.